Journal: Cancer Research

Article Title: Epithelial to Mesenchymal Transition Is a Determinant of Sensitivity of Non–Small-Cell Lung Carcinoma Cell Lines and Xenografts to Epidermal Growth Factor Receptor Inhibition

doi: 10.1158/0008-5472.can-05-1058

Figure Lengend Snippet: Figure 3. A, NSCLC lines were grown as s.c. xenografts in nu/nu mice and dosed for 14 days with erlotinib (100 mg/kg qd) or vehicle control. Statistical evaluation of percent tumor growth inhibition by repeated measures ANOVA was done. H292, H358, and H441 were found to be sensitive to EGFR inhibition in vivo. No correlation between tumor growth rate and sensitivity to EGFR inhibition was observed. Solid bars, erlotinib dosing period. B, tumor cell lysates were evaluated for the expression of epithelial and mesenchymal proteins. Tumors were grown to a volume of f300 mm3, excised, and flash frozen in liquid nitrogen. Tumor tissues were pulverized while frozen, subjected to detergent lysis and SDS-PAGE as described, and immunoblots probed with antibodies to E-cadherin, h-catenin, Brk, fibronectin, and vimentin. Similar with in vitro results, E-cadherin expression was restricted to erlotinib-sensitive lines and vimentin to insensitive lines. Antibody to GAPDH was used as a loading control.

Article Snippet: The human NSCLC lines (H292, H358, H322, H441, A549, Calu6, H460, H1703, SW1573, Calu3, Colo699, H2122, A427, H1437, H1299, Hop92, and H23) were cultured in the appropriate American Type Culture Collection recommended supplemented media.

Techniques: Control, Inhibition, In Vivo, Expressing, Lysis, SDS Page, Western Blot, In Vitro

Journal: PLoS Computational Biology

Article Title: Oxygen-Driven Tumour Growth Model: A Pathology-Relevant Mathematical Approach

doi: 10.1371/journal.pcbi.1004550

Figure Lengend Snippet: Panels (A-B) show fits of the model for Calu6 and Colo205. The plots also include simulation of hypoxia and necrosis. (A) is a faster proliferating tumour model (Calu6) and (B) grows slightly slower (Colo205). (C-D) CD31 IHC staining in Calu6 and Colo205 respectively. (E) summary data of CD31 for both tumour models.

Article Snippet: We stained tissue sections of the following tumours: Calu6 (n = 8), Calu3 (n = 7), one squamous cell lung cancer explant (n = 28) and squamous cell lung clinical cancers (n = 12) from AstraZeneca databanks.

Techniques: Immunohistochemistry

Journal: PLoS Computational Biology

Article Title: Oxygen-Driven Tumour Growth Model: A Pathology-Relevant Mathematical Approach

doi: 10.1371/journal.pcbi.1004550

Figure Lengend Snippet: (A) Growth curve fit for 4 explant models, 2 for squamous lung carcinoma and 2 for colorectal carcinoma. The xenografted cell line Calu3 shows very similar behaviour to explant models. (B) Comparison between Calu6 and Calu3 lung cell lines; a squamous lung cancer explant; and clinical tumour material analyses. The bar chart shows the proportions of microvessel density (MVD) in area (quantified from CD31), necrosis (quantified from Hematoxylin & Eosin staining) and stroma (alpha smooth muscle actin (αSMA) positive staining). (C) Images of the different tumour models stained for αSMA and counter-stained with hematoxylin.

Article Snippet: We stained tissue sections of the following tumours: Calu6 (n = 8), Calu3 (n = 7), one squamous cell lung cancer explant (n = 28) and squamous cell lung clinical cancers (n = 12) from AstraZeneca databanks.

Techniques: Comparison, Staining

Journal: PLoS Computational Biology

Article Title: Oxygen-Driven Tumour Growth Model: A Pathology-Relevant Mathematical Approach

doi: 10.1371/journal.pcbi.1004550

Figure Lengend Snippet: Parameter results ( k p and k R ′ ) for the ODM model in xenografts. Confidence intervals for each parameter are specified as well as the rank of the FIM (as a measure of the number of identifiable parameters), the collinearity index (Identifiable if γ <10), the condition number (Indentifiable if κ <1000) and the normalised residual. Cell lines denoted by * are unidentifiable and cell lines denoted by # are arguably identifiable.

Article Snippet: We stained tissue sections of the following tumours: Calu6 (n = 8), Calu3 (n = 7), one squamous cell lung cancer explant (n = 28) and squamous cell lung clinical cancers (n = 12) from AstraZeneca databanks.

Techniques: